State-of-the-art equipment to ensure the highest quality data
List of Instrumentation

List of Instrumentation

Protatgen Protein Services (PPS) offers an extensive range of analytical capabilities to support your protein project. Biopharmaceuticals such as various antibody formates, new biological entities (NBEs), peptides or other formates have no secrets for us: we have worked on over 300 diverse Biologics projects that emphasize our expertise. Our analytical services are customised to meet your project needs, from research over development to approval with custom analytical packages.

  • Analytical Capabilities
  • List of Instrumentation

Protein Quantification

  • Amino Acid Analysis (AAA)
  • Extinction Coefficient (A280)
  • Quantification by Protein-A HPLC
  • Isotope Dilution MS

Protein Modification

  • Deamidation (MS, cIEF)
  • Oxidation (MS, RP-HPLC)
  • Disulfide Linkage (MS)
  • Free Thiols (Ellman)
  • Truncations (cGE, MS)
  • C-terminal Lysine (cIEF ± CPB)

Aggregates

  • SEC-UV-MALLS
  • Analytical Ultracentrifugation (AUC)
  • Asymmetrical flow field-flow fractionation (AF4)
  • Visible and Subvisible Particles (LO/MFI)
  • cGE (non-reduced)

Protein Primary Structure

  • Peptide Mapping
  • Intact Mass
  • N-Terminal Sequencing (Edman)
  • De-Novo Sequencing

Protein Impurities/Variants

  • Process- and Product-Related Impurities by Charge (IEX, cIEF, CZE)
  • Purity and Product-related Impurities by Size (cGE non-reduced and reduced)
  • Protease activity (FRET Assay)
  • HCP analysis (ELISA, MS)
  • High-resolution 2D PAGE
  • Western Blot
  • Residual Host cell DNA (qPCR)

Higher Order Structure

  • Circular Dichroism (CD)
  • Infrared Spectroscopy (FTIR)
  • Fluorescence Spectroscopy
  • Differential Scanning Calorimetry (DSC)
  • Dynamic light scattering (DLS)
  • H-D-Exchange Analysis (HDX MS)

Glycosylation

  • N-linked Glycosylation
  • O-linked Glycosylation
  • Sialic Acid Content
  • Monosaccharide Analysis
  • Glycosylation Site
  • mAb NGHC and HC ratio    determination (reduced cGE)

Potency

  • ELISA
  • Cell-based Assays
  • Binding Assays

Miscellaneous

  • Apperance, Color, Clarity, Turbitity
  • pH, Osmolality, Density
  • Polysorbate quantification
  • Extractable volume
  • Content Uniformity of Dosage Units
  • pI determination (cIEF, IEF)

SERVICES

Protagen Protein Services (PPS) is a world leading CRO and recognized expert for analytical services in protein science regarding characterization, method development, validation and routine testing of antibodies, proteins, vaccines and other formates of biopharmaceuticals. Our sites are equipped with modern, state-of-the-art equipment for the analytical characterization of biopharmaceuticals regarding structure, purity, chemical modification, aggregation, particle formation, thermal stability e.g.. We offer service modules for analytcal support from research to approval regarding:

  • Developability
  • Clone Selection & Process Development
  • Extendend Characterization & Comparability
  • Stability & Release

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Protagen Protein Services (PPS) is a world leading CRO and recognized expert for analytical services in protein science. More than 20 years of market experience and the comprehensive spectrum of validated analytical methods ensure the highest quality for customers in the pharmaceutical, biotech and life science industry.

PPS supports Biosimilar developers with a broad range of analytical methods and consulting in achieving and demonstrating Biosimilarity

LATEST NEWS & EVENTS

Biotech Outsourcing Strategies (BOS) Basel 2019