IMG_4733Quantify a single protein in a crowd

An enzyme-linked immunosorbent assay (ELISA) takes place in a polystyrene plate that passively binds antibodies and proteins together. Once the components are immobilised, they are easy to observe.

Designing and performing ELISAs are simple when compared with cell-based assays. We add value to the process by making modifications to the basic technique, so that it is tailored to your specific requirements. For instance, we can demonstrate the specificity of the antibodies used in developing the assay by using our UNIchip® technology or 2D PAGE/Western blotting.

With ELISAs, it’s possible to quantify one specific protein and ignore all the others in the same sample. This is in contrast to amino acid analysis (AAA), where all the proteins are quantified indiscriminately.

Performing an ELISA, also known as an enzyme immunoassay, is a relatively simple way to do carry out quality control if you’re in the stages of drug purification, lot release or stability testing. We can quantify and identify single proteins in different matrices with great sensitivity and precision.

ELISAs can be done in a wide range of situations, such as during receptor-ligand binding (for example, in an antibody-antigen interaction) and when determining protein impurities.

To work out protein quantity, we find the extinction coefficient of your sample and combine it with amino acid analysis, as recommended by ICH Q6B.

Technical information

ELISAs can be applied to detect and quantify:

  • Specific analytes (the chemical substance which is the subject of analysis), by applying antibodies in a complex matrix. The major aim here is to demonstrate the specificity of the antibodies used in developing the assay
  • Process-related protein impurities, such as host cell proteins (HCP) or column leakage (protein A)

Quantitative and qualitative ELISAs are performed in microtiter plate (MTP) format using an enzyme linked detection system with an absorbance or fluorescent readout.

Impurities such host cell proteins (HCPs) can be detected using high resolution 2D PAGE or mass spectrometry.

Your contact for ELISA at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com